Nandan Amol Merchant

3 Questions 2 Answers 0 Followers

Questions related from Nandan Amol Merchant

Western blot for amyloid beta 42 protein? Best lysis buffer and conditions?

I am treating neuroblastoma cells with AB42 protein (monomers and fibrils in separate experiments) and studying what happens after its uptake and concurrent interactions with intracellular...

19 March 2019 4,569 3 View

How do we sterilize amyloid beta 42 protein solution for cell culture?

I will be treating SHSY5Y cells with amyloid beta 42 obtained from E. Coli cultures. I observed that after treatment, the media became cloudy within 24 hours indicating a contamination. I did not...

06 December 2018 3,560 2 View

Can neuroblastomas grow on SiO2?

As initial iterations, I am trying to grow the neuroblastoma cells SHSY5Y cells on a glass (SiO2) surface of a microfluidic device without any ECM treatments. Will focal adhesion proteins form...

06 December 2018 165 0 View