Nancy E Corral-Fernandez

3 Questions 8 Answers 0 Followers

Questions related from Nancy E Corral-Fernandez

Problem with CaM antibody from Cell Signaling?

Hello I am currently trying to get a specific band for CaM (17kDa), according to Cell Signaling; the antibody which the lab bought, I should use: 1:1000 for primary dilution in 5% BSA in TBST all...

06 June 2019 4,707 3 View

Problems with loading the amount of protein in Western Blot?

Hi. Recently I began to extract protein from different rat organs: brain, lung, salivatory glands... using RIPA buffer and sonication. I use BCA for cuantification of protein, and I`m getting...

05 May 2017 3,596 5 View

Primary antibody in 5% skin milk-TBST long-term storage?

I been trying different concentrations using PBS+Sodium azide+BSA and only works in 5% skin milk. I read that you can add sodium azide to the skin milk? Can I freeze it at -20ºC? or leave it at...

09 September 2016 5,134 8 View