Nancy E Corral-Fernandez

4 Questions 11 Answers 0 Followers

Questions related from Nancy E Corral-Fernandez

Problem with CaM antibody from Cell Signaling?

Hello I am currently trying to get a specific band for CaM (17kDa), according to Cell Signaling; the antibody which the lab bought, I should use: 1:1000 for primary dilution in 5% BSA in TBST all...

05 June 2019 4,357 3 View

Problems with SCB Protein Standard?

Hello. We recently bought Cruz Marker Molecular Weight Standards from Santa Cruz Biotech and for some reason, we only see one band (we think is the 90KDa, but since we can not see the others...)...

07 August 2017 2,936 7 View

Problems with loading the amount of protein in Western Blot?

Hi. Recently I began to extract protein from different rat organs: brain, lung, salivatory glands... using RIPA buffer and sonication. I use BCA for cuantification of protein, and I`m getting...

03 May 2017 5,692 4 View

I´m working with western blots, however all my blots are getting dark. I´m using the Licor Westernsure ECL substrate. Any ideas?

I use the ChemiDoc XRS+ BioRad for imaging Primary antibody 1:1000 Secundary antibody 1:20 000

17 August 2016 9,206 17 View