Nana Shimamoto

2 Questions 1 Answers 0 Followers

Questions related from Nana Shimamoto

Is EGFR needed for the primary culture of cancer associated fibloblast?

I cultivate CAF from the specimen of the pancreatic cancer patient who had pancreaticoduodenectomy (PD). Other than the medium, FBS, antibiotics, is EGFR needed for the culture?

08 April 2024 8,926 0 View

How do you conduct SiRNA transfection to pancreatic cell lines using lipofectamine 3000?

I am trying transfection of SiRNA of COPB2 to pancreatic cell lines in 96 well. Bxpc-3 is difficult to transfect SiRNA following the manufacture's protocol (lipofectamine 3000). So, I have...

27 December 2023 4,028 1 View