Nana Abena Anti

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Can you use Ficoll gradient on RBC-lysed and frozen cord blood white blood cells?

I have several frozen white blood cell samples from human cord blood that I would like to isolate neutrophils from whiles keeping the rest of the white blood cell fractions. I know that the Ficoll...

27 April 2023 658 0 View

Has anyone experienced degradation of their proteins from white blood cells or PMBCs when they perform a western blot?

I have performed several western blots using lysates from umbilical cord white blood cells that have been treated for about 48hrs and lysed with RIPA buffer (Halt protease inhbitor, 10mM PMSF and...

11 November 2022 3,556 1 View