6 Questions 16 Answers 0 Followers
Questions related from Namratha Sastry
I'm studying a gene whose expression is regulated by DNA methylation. Cancer cell lines that express this gene show strong hypomethylation, while cells lines that don't express it show...
20 March 2019 7,957 2 View
I recently found that the levels of pERK increase when I infect glioma stem cells with my protein of interest. I'm trying to determine which receptor (specifically RTK) may be responsible for this...
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I'm trying to do a co-IP looking at a GPI-anchored protein and a receptor. I want to be careful while selecting a lysis buffer, to ensure that I don't disrupt the interaction. Can anyone recommend...
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I'm studying a GPI-anchored protein and all of the antibodies against this protein are ~5-7kD higher than the expected molecular weight (expected is ~18kD and most antibodies give ~25kD). I'm...
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I'm trying to do an invasion assay using glioma stem cells and I'm trying to determine my options. I found that two common methods of testing invasion are the matrigel invasion assay and the...
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I'm trying to do a lentiviral transfection/transduction of U87 glioma cells. I transfected my vector into HEK283FT cells (packaging cells) and I wanted to use the viral media on the U87 cells. The...
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