Najeeb Ullah

3 Questions 7 Answers 0 Followers

Questions related from Najeeb Ullah

Protein Extraction, Heterodimeric protein Lysis Buffer Composition?

I am working on a heterodimeric protein expressed in same E.Coli, two different lysis buffers are used for their purification. i.e. 50 mM NaH2PO4, pH 8, 300mM NaCl, 10mM imidazole, and 50mM...

10 October 2017 9,238 4 View

For 94kDa Cellulase i will use Strep-tag and TEV cleavage, without C-terminal-His, where to cleave and construct it in expression vector pET-52b(+)?

protein expression, extraction, purification, and crystallization.

09 September 2017 5,915 2 View

What is the possible role of disodium citrate in protein dimerization as i am going to use for protein dimerization but i didnt find any relevant lit.

protein, protein dimerization, buffers role, salts role in proteins precipitation, proteomics, protein crystallography

07 July 2017 3,395 8 View