Naina Singhi

2 Questions 5 Answers 0 Followers

Questions related from Naina Singhi

Does protamine sulfate improve lentivirus mediated transduction?

Hello, I'm planning to use protamine sulfate to infect mouse fetal liver HSCs with lentiviral vector on retronectin coated wells. The goal is to see if it improves the lentiviral transduction...

13 February 2018 4,264 11 View

Does polybrene cause clumping in Fetal Liver HSCs during lentiviral transduction?

Hello, I'm trying to transduce fetal liver cells using lentivirus, and I'm using polybrene @ 8ug/mL to enhance the transduction. I noticed that the cells become clumped soon after being exposed...

30 November 2017 1,423 3 View