Nahal Rose Lalefar

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Questions related from Nahal Rose Lalefar

Should there be no adherent cells when you culture murine LSK cells?

I have been culturing LSK cells isolated from C57BL/6J mice (ages ~8-12 weeks) after sorting using base stem cell media (with 10% FBS, 2-ME, MβCD) + SCF (5-25ng/ml). I am trying to look at...

27 October 2014 7,763 6 View

How many LSK cells would be expected from a single C57Bl/6J mouse?

I am harvesting BM cells from bilateral femurs, tibias, pelvic bones and humeri from WT mice ages 8-12 weeks, followed by c-kit magnetic bead enrichment and then flow cytometry to sort out the LSK...

18 July 2014 9,756 8 View