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Questions related from Naghmeh Azadfar
I am annealing 37 DNA oligos together but i would like to stop it in certain time. i tried to add loading dye to stop it but it did not worked. Do you know how can i stop it?
15 December 2015 7,736 5 View
I am purifying a His-tag fused protein by Äkta instrument from GE Healthcare. The column in 1 ml HisTrap column. after loading the lysis to the column and washing by washing buffer, i elute the...
03 July 2015 8,628 12 View
I purified a mRuby2 protein from Bactria. the extinction coefficient of this protein is 113000 M-1cm-1. but my expressed mRuby2 protein has a extinction coefficient of 45000 M-1cm-1. what would be...
09 March 2015 6,534 12 View
I have a fluorescent protein in a pBAD vector and I want to express and purify it. At first, I cultured a colony in 100 ml LB medium at 37 °C for over night. Then, I inoculated 25 ml from over...
20 February 2015 5,685 9 View
I assembled a DNA origami, a 6HB, and I run a scaffold and assembled 6HB in a agarose gel. In a gel, the band of scaffold is higher than 6HB band. actually, the molecular weight of 6HB is more...
28 August 2014 1,127 2 View
Hydrophobic peptide (53%), 15 a.a.-long contains a single Cys-residue and Amine free, can be dissolved in DMF. I need to attach Alexa Fluor647-maleimide to the thiol group of the peptide and Alexa...
25 July 2013 8,846 7 View
I have a sequence of my construct in CLC Sequence Viewer and I want to change the name of annotation and add another annotation to the construct sequence. Does anyone know how can I do...
12 July 2013 3,713 1 View
