Nagabushan Reddy

2 Questions 8 Answers 0 Followers

Questions related from Nagabushan Reddy

Does anybody have an experience with the cell line constantly expressing luciferase system, if so, would it affect the biology of the cells?

I'm planning to construct a vector with dual luciferase system and establish a 3T3-L1 cell line. However, I have some doubts if the constant expression of the luciferase would effect the...

08 August 2015 9,819 4 View

Lentiviral Transduction - not working ! Can somebody tell me what's wrong?

Hi all, I'm producing lentiviral particles using third generation vectors from add gene using second generation packaging plasmids (which is also compatible) , I use RT-qPCR for titrating...

09 September 2014 9,516 17 View