Nagabhushanam Reddy

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Questions related from Nagabhushanam Reddy

Protein precipitation issue in elution of Anion exchange chromatography

Dear all, Anyone can suggest me regarding a precipitation issue in elution of Anion Exchange chromatography, After elution thread like particles are forming in elution pool. We are using PB...

20 June 2017 5,233 9 View

Hi everybody, Please suggest me how to stop protein aggregation or precipitation at final stage?

My protein after column purification performing buffer exchange at 10kDa membrane with formulation buffer (50mM PB+ 100mM NaCl + 0.25mM EDTA, pH 7.3 & Cond.: 16mS/cm). After buffer exchange i...

01 January 1970 499 2 View