Nafiseh Sanei ata-abadi

3 Questions 13 Answers 0 Followers

Questions related from Nafiseh Sanei ata-abadi

Can I isolate two supercoiled plasmids from each other by HPLC?

I want to isolate to supercoiled plasmid from each other. I don't know is it possible by HPLC? and I don't know which column should I use? does anyone have such this experience?

09 September 2014 6,582 7 View

Does anyone have experience with purification of secreted his tagged proteins from a condition medium OF HEK293T cells?

I want to purify my secreted his tagged protein from condition medium of HEK293T cells using HIS SELECT AFFINITY GEL OF SIGMA - BATCH method (NOT column chromatography). Does anyone use this...

05 May 2014 851 3 View

What level of saturated ammonium sulfate should I used for BSA precipitation?

I want to purify a secreted, HIS-tagged 24 kD protein from a culture supernatant of Hek 293T cells. If I wanted to remove the BSA from the medium, what level of saturated ammonium sulfate should I...

05 May 2014 3,135 5 View