QPCR probe optimization troubleshoot - big CT value, and no ct value in the qpcr result with WT and homozygous confirmed sample. Valid or error?

Im doing a qpcr for genotyping ERCC1 rs11615, now Im in a middle of optimization of Probe ERCC1 wild type, with 2 confirmed sample (WT and heterozygous sample). and the result, Ive got a big CT...

23 November 2023 9,959 4 View