Nadine Kleinhans

5 Questions 4 Answers 0 Followers

Questions related from Nadine Kleinhans

What is causing these bright bands on my RNA gels?

Hi all, So I have been doing high-throughput RNA extractions on xylem from Eucalyptus hybrids (urophylla x grandis). My extraction combines a custom CTAB protocol with silica column binding and...

04 October 2023 8,864 1 View

Experience with RNAlater-ICE influencing transcriptomic data?

Hi all, I am developing a high-throughput RNA extraction protocol for xylem vessels. Pre-emptively, the samples are going to be homogenized in a Genogrinder with a cryoblock attachment and then...

16 February 2022 8,575 1 View

E.coli cells look lysed after spin down for competent cells?

Hi all, So I am continuously experiencing a very weird phenomenon (see images attached). I am trying to get E.coli DB3.1 cells competent together with SURE and XL-Blue cells. I always streak out...

28 June 2021 2,508 5 View

Laemmli buffer. I used trypan blue instead of bromophenol blue. Will it affect my proteins?

Hi All We ran out of Bromophenol blue in the lab. Is it plausible to use trypan blue instead? I plan on using the proteins separated on the SDS-PAGE gel downstream in a Western blot. Will it...

09 February 2018 7,036 5 View

PKNG101 Nucleotide Sequence. Does anybody have it?

Hi all Another pKNG101 question. I have successfully optimized my restriction digests, but now I am getting multiple bands when digesting with SmaI. I have a suspicion that the plasmids stocks...

07 August 2016 4,602 1 View