Nadine F Joseph

3 Questions 3 Answers 0 Followers

Questions related from Nadine F Joseph

How to analyze dendritic spines from tissue (Prelimbic cortex)?

Hi there, I am trying to measure dendritic spines from mouse fixed tissue, specifically, I am looking at the prefrontal cortex. I injected the PrL with a lentivirus to knock-down my protein of...

28 January 2021 9,605 2 View

How to prevent being offset of target when bilaterally cannulating the medial PFC of mice?

Hi All, I have been running into a technical issue when I try to cannulate the prelimbic cortex of mice. I find that I am about .1-.2 off from being even (see the attached pictures). I am using...

13 March 2018 9,285 3 View

Has anyone gotten GAD67 (ab26116) and CAMKIIα (ab87597) to work for IF in mouse free-floating sections?

Hi All, I am optimizing an IF protocol for GAD67 (ab26116) and CAMKIIα (ab87597) double labeling in free-floating sections from the mouse brain. I can't get a signal for the life of me. I tried...

07 December 2016 2,424 4 View