How may I clone a 5415 bp gene into pglo plasmid (5371 bp) replacing it's GFP gene (~700 bp)?

I need to clone a 5415 bp gene into pglo plasmid replacing it's GFP gene which is around 700bp long. Now PCR amplification of this 5415 bp gene using PhusionR HiFi Taq doesn't show any band, only...

06 October 2019 5,046 7 View