Nadia Tin Nur

2 Questions 3 Answers 0 Followers

Questions related from Nadia Tin Nur

How may I clone a 5415 bp gene into pglo plasmid (5371 bp) replacing it's GFP gene (~700 bp)?

I need to clone a 5415 bp gene into pglo plasmid replacing it's GFP gene which is around 700bp long. Now PCR amplification of this 5415 bp gene using PhusionR HiFi Taq doesn't show any band, only...

06 October 2019 5,070 7 View

Can a protease gene be responsible for both cleaving off and transporting the cleaved peptide outside cell?

I'm working on Staphylococcus hominis epcidin producing gene.Now the protease is responsible for cleaving the leader sequence off the peptide,then it'll be transported out of the cell.Now I can't...

28 July 2019 7,490 0 View