Hi, quick question on collecting cells to lyse for westerns. Cell pellet was sitting in media at RT for ~30min. Will this cause protein degradation?

I trypsinized the cells, incubated 5 min. washed with media and spun down, all at RT, as if to split them. After spinning, however, I just left them as a pellet  in media while I finished my...

01 January 1970 7,410 4 View