Nadezhda Stavtseva

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Questions related from Nadezhda Stavtseva

What could be the reason of unstable cell parameters in cell mode (for voltage clamp recordings)?

Rseries, Rmembrane and Cmembrane in cell mode seems to be unstable. They are changing, sometimes even to negative values. What could be the reason?

26 October 2022 1,618 2 View

Which osmolarity difference between internal and external solutions is optimal for whole-cell patch clamp recordings?

I just started to learn how to do a patch clamp and had difficulties to obtain a gigaseal with ACSF 290 mmol/kg and Cs-based solution with osmolarity 310-317 mmol/kg. When tried to do it after...

31 August 2022 4,630 1 View