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Questions related from Nada Mohamad
I´m trying to do a DNA/Protein Gel Shift Assay using agarose gel to check if my protein is binding DNA or not. The problem is the PI of my protein is high (around 9.5) so the sample won't run...
10 October 2014 1,589 4 View
Note: We have a MES Sodium Salt reagent from Sigma and another one of MES Hydrate but dunno which one to use and how?
08 August 2014 8,241 3 View
I recently cotransformed a plasmid that contains my gene of interest with Groel/S Chaperones in BL21 (DE3). After I got so many colonies I expressed in 1L of LB and induced using o.5mM IPTG 20...
06 June 2013 5,579 2 View
So the story is that my protein is taged with MBP. I tried to purify it both on 5 ml and 1 ml columns but the result was bad! It had very dirty flowthrough and wash, and the elution was with a...
05 May 2013 8,931 5 View
I have been trying to purify a 76 kDa protein and it was a hard process. I tried His tag but I didn´t get good results but when I used it with MBP I had a very good result and big bands after...
05 May 2013 7,896 4 View
The proteins I am working on are 75.9 kDa with pI around 10.2 and 67.3 kDa with pI around 9.5 respectively and i´m am trying to find the perfect buffers (PH, composition, etc..) to use in...
04 April 2013 5,384 16 View
