Nada Mohammed Saleh

2 Questions 4 Answers 0 Followers

Questions related from Nada Mohammed Saleh

Could the small transmembrane domain of the recombination protein from the virus be the reason for the lack of expression in E. coli?

I have a protein that is 117 amino acids long, with a 21 amino acid transmembrane domain at the C-terminus. I've cloned this protein into the pET28 expression vector in BL21, but am not seeing any...

21 May 2024 8,119 2 View

Why I have 2 bands after PCR and gel purification?

I am amplifying gene (1488 bp) from RNA genome using specific primers and run in reverse transcription PCR(RT-PCR). The result, I am getting 2 bands my target band (1488bp) and extra band with...

02 September 2023 4,953 7 View