3 Questions 5 Answers 0 Followers
Questions related from Nada Mohamad
Note: We have a MES Sodium Salt reagent from Sigma and another one of MES Hydrate but dunno which one to use and how?
14 August 2014 9,904 3 View
I recently cotransformed a plasmid that contains my gene of interest with Groel/S Chaperones in BL21 (DE3). After I got so many colonies I expressed in 1L of LB and induced using o.5mM IPTG 20...
28 June 2013 1,466 2 View
The proteins I am working on are 75.9 kDa with pI around 10.2 and 67.3 kDa with pI around 9.5 respectively and i´m am trying to find the perfect buffers (PH, composition, etc..) to use in...
05 April 2013 6,840 16 View