Re-solubalization of protein pellet after MeOH-CHCl3 precipitation?

I am doing the click reaction on the HEK293T cell lysate (200ug proteins) for 1h at R.T. After the click I perform the MeOH-CHCl3 precipitation, then I try to re-solubalize the pellet in 4% SDS...

02 August 2023 3,357 4 View