Nabeel Farooqui

3 Questions 3 Answers 0 Followers

Questions related from Nabeel Farooqui

I have some problems with Tris-Tricine SDS PAGE. Any one knows about it ?

Hey everyone! As I run my hydrolyzed samples in the Tris-Tricine buffer system, the bands were disappeared as the samples run into the gel. Lane 1 = Marker (10 - 180KDa) Lane 2 = BSA...

21 October 2019 6,523 3 View

Hey everyone ! I need to extract protein from Sea cucumber Anyone have protocols ?

Hello !!! I have tried many different methods for protein extraction from Sea cucumber (Apostichopus japonicus) i.e. Acid treatment, Alkaline methods, and TCA precipitation along with ethanol,...

22 September 2019 3,342 2 View

My question is about transfection of Bel 7402 cells. After trying knockdown, the cells were not expand and washed away by 50% when I changed media?

Every time my cells remains 50% after changing the media followed by gentle wash with PBS, and just particles surround in the media after overnight incubation in 5% CO2 incubator.

27 June 2019 6,190 2 View