Muzammal Hussain

2 Questions 3 Answers 0 Followers

Questions related from Muzammal Hussain

How to deal a kinase protein precipitation after Nickel elution?

Hi, I am trying to purify a kinase protein (theoretical pI = 9.5) by HIS-select Ni-affinity column, washing with 50mM Tris HCl (pH 7.5), 0.5M NaCl, 20mM imidazole (buffer A) and a gradient elution...

01 January 2018 8,187 2 View

Ligand-protein Steered MD using Amber?

Hi, Is there any protocol to conduct Steered MD simulations for ligand-protein complexes using Amber?

01 January 2018 3,714 0 View