What happens if I don't insert any sgRNA-coding sequence into the CRISPR/Cas9 plasmid carrying sgRNA backbone? What will be expressed in such case?

The specific plasmid I am asking about is pSpCas9(BB)-2A-Puro (PX459) V2.0. Imagine that I do not insert any guideRNA-coding sequence downstream to the U6 promoter inside this plasmid, will there...

08 July 2015 3,418 14 View