Muthuramalingam Karpagavalli

2 Questions 3 Answers 0 Followers

Questions related from Muthuramalingam Karpagavalli

How different the plots would be for RT-PCR data, 1) normalized wrt to internal control vs 2) showing variance in siRNA-control and plotting?

I have RT-PCR data of siRNA silencing in order to check the expression of other genes wrt to the silenced gene. When I normalized the data wrt internal control (U6, 18S RNA) and used the student...

01 December 2023 511 2 View

How to take a common CpM value for different RNA seq dataset of different samples?

I downloaded the RNA-Seq dataset from the depository and took it for analysis. For a tissue, sequencing was done 12 times and therefore had different sets of reads and normalized counts. Now for...

10 November 2022 4,517 2 View