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Hi to everyone, I am currently working on western blot for a lower Kda protein ranges around 30-50, i am using 70v and 90mins for transfer at room temperature but still the protein remains in gel...
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Want to study about these enzyme hence it becomes vital to know the enzyme thoroughly, but I couldn't find any data regarding the cells which lacks this enzyme.
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I am working on animals with a new compound. Already I have worked on cell lines with the same compound. How can I fix my doses for animal study using my cell lines data.
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