Murugappan Sathappa

2 Questions 11 Answers 0 Followers

Questions related from Murugappan Sathappa

Clipped protein products during purification (even with protease inhibitors added)

I am trying to express and purify a human protein in E.coli (His6-SUMO fusion tag). The total protein size is 67kDa with the fusion tag and the protein of interest is 47kDa. The expression is...

07 July 2017 3,964 6 View

Why is His tag not binding to Ni, or are there any non-specific interactions?

I am trying to purify my His-tagged subunit of a protein complex using a Ni-NTA agarose column. I am doing a WT control with no His tag on the subunit of protein complex. Based on Western Blot,...

10 October 2013 2,223 27 View