Murtala Muhammad

2 Questions 6 Answers 0 Followers

Questions related from Murtala Muhammad

Which PCR protocol will be suitable for primer with high TM of 70C and 26bp?

Hi, I am trying to mutate my gene of interest by PCR and then to transfer in competent Cell DH10b , My primer have length of 26bp and TM 70.14C , I have set the TM of 55C for my primer in...

11 November 2016 981 4 View

How to sovle the problem of secondary structures annealing in site directed mutagenesis ?

Hi, I am new to site directed mutagenesis, my teacher design primers for site directed mutation having TM of 70.14C ,57.03C,53.89C and 59.69C respectively . I have made master mix for my PCR with...

11 November 2016 722 5 View