6 Questions 8 Answers 0 Followers
Questions related from Munirah Faleh
I’m seeking expert advice from anyone with experience in protein purification. My project involves depleting fibronectin (FN) from fetal bovine serum (FBS) to use the FN-depleted serum in routine...
17 July 2025 5,797 3 View
Hello everyone, I would like to hear experts' opinions on this matter. I am working on gene knockout using CRISPR-Cas9, but unfortunately, I did not successfully achieve my target gene insertion...
14 March 2025 9,705 2 View
I am attempting to prepare a glycerol stock for Stbl3 cells from commercially competent cells for Stbl3. I followed these procedures to prepare the glycerol stock, and I would greatly appreciate...
29 January 2025 8,477 3 View
Hi everyone, I'm hoping to connect with some experts on the HepG2 cell line who can share their insights on working with these challenging cells. I'm currently trying to seed them in 6-well plates...
06 January 2025 5,296 6 View
I am working on Zika virus propagation and have conducted this process multiple times. Each time, I increased the viral input to the cells, but unfortunately, I consistently obtained low virus...
03 December 2024 4,764 2 View
In my study of Zika virus pathogenesis, I propagated the virus by inoculating 100 µL of virus stock into 80% confluent Vero cells. Cytopathic effect (CPE) was observed on day three...
04 October 2024 3,106 0 View
