Mukesh Sankar

4 Questions 7 Answers 0 Followers

Questions related from Mukesh Sankar

How to identify an unknown gene responsible for the synthesis of certain metabolite in E. coli?

The (known) genes responsible for certain metabolite production were deleted. But, we still see the production of same metabolite in significant amount during glucose fermentation in E. coli.This...

27 February 2018 3,272 2 View

Can anybody suggest me the concentration of ammonia needed to be used for pH control in fed batch fermentation?

I am working on production of acid metabolites in E.coli in which I have to compare the effect of various bases like NaOH, KOH and Ammonia etc. Could anybody please suggest the concentration of...

19 June 2013 2,050 13 View

Growth characteristics of E. coli RR1: can it be grown on minimal media containing glycerol as a carbon source?

I am studying the growth and acid tolerance of various E. coli strains. I am growing cells in minimal media containing glycerol as a carbon source. During this study, I have noticed that E. coli...

24 April 2013 5,616 4 View

I have over expressed a small protein (21 amino acids) in plasmid. Are there any methods to check its expression? The size of the protein is 2.3 Kda.

When i try to check its expression on 18% SDS gel, i could not find any band. Could anybody suggest a method ?

04 January 2013 7,967 7 View