Mujtaba Allawati

2 Questions 4 Answers 0 Followers

Questions related from Mujtaba Allawati

Weird sequencing result?

Hi colleagues; Can any one help with attached image of Sanger sequencing, where Alignment result shows SNP cant be confirmed by the other primer ( 2 nucleotides difference). One primer shows SNP...

28 July 2019 3,147 4 View

Can you share your experience on weird qPCR melting curve peak?

Hello, I have been running qPCR (on Qiagen rotor-gene machine) to amplify one of the RHD gene exons with perfect melting curve peak. A sudden weird melting curve peak appeared with last runs. To...

12 June 2018 2,377 2 View