Muhd S

2 Questions 4 Answers 0 Followers

Questions related from Muhd S

Is the filtering of cells (using the cell strainer) necessary before tail vein injection into mice in spontaneous metastasis experiments?

The experiments involve the breast cancer cell line MDA-MB-231. Just wondering if clumps of cells that may occur after trypsinisation during preparation may affect the distribution of cells to the...

13 January 2014 4,440 3 View

If my transduction efficiency is high enough, can the transduced cells be used for xenograft implantation without the need for subcloning, etc.?

More information: The transduced sequences include GFP and antibiotic resistance. Or, can I simply subject the cells to a period of antibiotic selection and then harvest them right away for...

18 January 2013 3,874 6 View