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I am doing genotyping to identify mutants. i use HD2B gene primer which are not working very efficiently. they work some times for some of the samples to barely amplify the gene (see figure...
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I did gateway cloning and transferred my constructs into GV3101. I did several PCR confirmations of GV3101 and made glycerol stocks and stored at -80. After about 3-4 months. I am unable to...
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