Muhammad Jamal

3 Questions 3 Answers 0 Followers

Questions related from Muhammad Jamal

Do I need to starve the cells before doing the glucose uptake assay in A549 cells?

Hello researchers, I am trying to check the effect of a chemokine on lung cancer cell (A549) metabolism. Last time I tried to induce the cells with different concentration of the chemokine...

09 September 2019 5,321 1 View

How the CRISPR-Cas9 increase the efficiency of DNA double stranded repair?

why the frequency of CRISPR-Cas9 mediated HR is always more than the normal HR in a cell/bacteria?

06 June 2017 3,084 1 View

How to increase the transfection in HEK 293 T cells?

I am co-transforming two plasmids (both 8kb) into HEK cells using Lipofectamine 2000, followed by infection, but each time the efficiency of transfection is very low (5-10%) and the more cell...

04 April 2016 9,799 4 View