Muhammad Faheem

6 Questions 20 Answers 0 Followers

Questions related from Muhammad Faheem

Enzyme pH optimization protocol?

I am optimizing pH for my cellulase enzyme with substrate CMC and pNPG. Can anyone provide me with a good protocol for pH optimization?

10 October 2013 3,289 6 View

Protein purification and enzyme activity?

I am working with cellulases, I have expressed my cellulase enzyme in pET28a expression vector. The bacterial lysate has activity against cellulose substrate but after purification of protein with...

09 September 2013 2,409 9 View

His-tagged protein purification?

Could anyone suggest me a good protocol for purification of my his-tagged protein. I am eluting my N-terminus his-tagged protein with imidazole but after purification my protein has no more...

09 September 2013 3,377 5 View

Any suggestions regarding purifying my protein?

I am purifying my protein with GE his-trap 1ml column, I have started with different imidazole concentration. I have my final concentration wast with 110mM imidazole wash and elution with 250mM. I...

08 August 2013 7,594 1 View

His-tagged protein purification with GE Hi-trap 1ml column?

I am purifying my his-tagged protein with GE His-trap 1ml column. I started with equilibration of the column with 10mM and 20mM imidazole, and washing has been raised to 50mM, 100mM, and 110mM...

08 August 2013 2,709 9 View

I have to double digest my gene in a Plasmid, but the buffers are not compatible, what should I do?

01 January 2013 3,941 12 View