Muhammad Akbar Shahid

6 Questions 10 Answers 0 Followers

Questions related from Muhammad Akbar Shahid

How to interpret dS/dN ratios?

Normally dN/dS ratios are calculated and interpreted as below one negative selection, above 1 is positive selection and 1 means neutral selection. How to interpret dS/dN ratios? Programs like SNAP...

06 June 2017 4,011 9 View

Is it compulsory to first use HRM calibration kit for performing HRM on CFX96 Real Time machine?

To perform HRM analysis for genotyping on CFX96 machine for the first time, BioRad recommends to use HRM calibration kit along with Precision melt analysis software. I am just wondering whether...

12 December 2015 7,089 7 View

Could someone give me some urgent information regarding HRM analysis software?

I have been using Corbett Rotorgene 6000 for HRM analysis. In my new lab, we have BioRad CFX96 machine but its software (Precision melt analysis) is very expensive (5000 USD) unlike rotorgene...

12 December 2015 452 7 View

Is standardizing protein concentration, either based on a few or multiple selected bands on SDS-PAGE, an acceptable method by peer reviewed journals?

After adjusting concentration of whole cell proteins from different bacterial strains, I want to measure relative expression of a particular protein by Western blot (DAB, Diaminobenzidine method)....

05 May 2014 1,258 6 View

Is it OK to use 2-D quant kit with protein sample lysis buffer containing glycerol and Bromophenol blue?

We normally use 4X protein sample lysis buffer containing 250 mM Tris (pH 6.8), 40% glycerol, 10% β-mercaptoethanol, 9.2% SDS, 0.02% bromophenol blue. I have used 2-D quant kit (GE, Health care)...

05 May 2014 7,372 4 View

How to normalize whole cell protein concentration using SDS-PAGE for different strains of a bacteria for which no internal control is available?

I want to determine relative expression of a particular protein by immunostaining/ western blot, using Diaminobenzidine (DAB) method, in mycoplasma cells for which, as such, no internal control is...

05 May 2014 8,086 3 View