Muhammad Zahoor

4 Questions 5 Answers 0 Followers

Questions related from Muhammad Zahoor

Plasma cells from healthy donors?

Dear researchers, I wanted to do one qPCR with Plasma cells from healthy donors. I am living in not very big city, so difficult to obtained bone marrow or tonsils from healthy donors. Therefore, I...

30 March 2017 6,956 2 View

Any one have experienced with western blotting for Hif1a?

Hi, i am having trouble with immunoblotting for HIF1a, and would like to have some tips from you guys. i have tried 4-12% SDS gel, dried/fast and wet transfer both, using Anti-Hif1a from R&D...

12 August 2016 3,108 12 View

DAB kit for goat primary ab?

Hi, i am interested to do immunohistochemistry (DAB staining) on mouse tissue, and looking if some one can suggest me any good kit. I do have primary antibodies from goat against the protein i am...

18 December 2015 6,084 4 View

How to seperate or purify the double allele KO cells from single allele KO cells, using CRISPR/Cas9?

I am using CRISPR/Cas9 system to KO a gene and the plasmid carries GFP as label. However, I am not sure how would I be able to differentiate or purify the double allele KO cells from single allele...

19 June 2015 8,223 7 View