Why i am not getting the actual band of TDtomato (1431pb) insertion after replacing GFP in PLL3.7 for sgRNA KI construct?

I am replacing GFP with TDtomato for my sgRNA KI construct. First, I performed a PCR for TDtomato using a TDtomato plasmid as the template. When I ran the gel, I observed two bands (700 bp and...

18 July 2024 6,206 2 View