How to Amplify Larger fragments (22.5 kb) and doing Gibson Assembly?

I am working on amplifying larger fragments (20.5 kb & 22.5 kb) using Phusion Polymerase and Q5 Polymerase. I am unsing isolated genomic DNA of Corynebacterium glutamicum as template. I am...

30 June 2025 4,986 1 View