Muhammad Awais

8 Questions 9 Answers 0 Followers

Questions related from Muhammad Awais

Persistent issue in NO GFP Bands in western blot. why?

Hello everyone, I’ve been working on expressing a fungal pathogen gene in tobacco leaves via Agrobacterium-mediated transient expression. Despite extensive troubleshooting over the past three...

09 June 2025 902 5 View

How to design primers for fungal and plant gene cloning?

How do we design fungal and plant gene cloning primers using BamHI and SalI restriction sites? What other additional sequence sites should be included and what about the gene sequences? Should we...

27 March 2025 9,009 2 View

Soil and root microbes, enzymes etc.?

I hope you all are in good health.. I required books and articles to study soil and root microorganisms. any suggested books or articles for a beginner to study these topics...

28 April 2023 3,763 2 View

If we add organic matter in highly acidic soil what will be the results?

in most of the cases we studied that if PH increases the organic matter will decrease similarly with decrease of ph, OM will increase... what will be the results if we increase organic matter by...

17 March 2023 339 3 View

Need a lab manual method for free aluminium determination in soil samples?

help me to suggest an article or lab method for the determination of free aluminum in soil samples.

01 January 1970 9,283 2 View

For writing a review of literature, can we use portions other than abstract like discussion or conclusion portions of a paper?

For writing a review of literature, can we use portions other than abstract like discussion or conclusion portions of a paper? introduction, discussion, or conclusion sections...

01 January 1970 8,491 2 View

Is it possible that pathogenic fungi lose their virulence after subculturing for a longer time?

Actually, I am working on a fungal pathogen Verticillium dahliae. I noted that the strain's growth and microsclerotia development are not as good as it was a few months ago, I have the same...

01 January 1970 2,035 6 View

Why the bands are not showing according to their bp lengths after PCR?

I extracted RNA using a plant RNA extraction kit and then converted it to cDNA using another kit. I used GFP-tagged plant gene primers to amplify those genes with a 50 µl total reaction mixture. I...

01 January 1970 3,042 1 View