Muhammad Aslam

5 Questions 10 Answers 0 Followers

Questions related from Muhammad Aslam

Can someone guide me how to transport Live Ciliates samples in a transport medium?

I want to transport Ciliate samples which will take 4-5 days. I would like to ship these alive.

22 February 2017 9,044 6 View

I was trying to isolate Lactobacillus DNA through boiling method, but got fragmented band. Why is it so, anyone kindly guide me?

I was trying to recover the Lactobacillus DNA through simple boiling method. Some of the samples failed but one showed fragmented band. The boiling method include: Loopful colonies from MRS media....

15 December 2016 3,406 3 View

I've tried to recover DNA isolation through FastPrep Soil Kit but failed to recover it twice. Can anyone please guide me how to solve the problem?

First directly spun the sample at took the sediment from Glacier and proceed it according to FastPrep Soil Kit protocol. Secondly, i treated the sample with Guanidine thiocynate, Tris-HCl and 10%...

04 December 2016 9,085 3 View

How can we Lyophilize Snow samples?

What is the effective way to preserve the Microorganisms during the process? As it will reduce the quantity and will be much easier to transport the viable temperature sensitive...

29 November 2016 6,918 8 View

How can we target Bacterial community in Glacier snow using Fast Prep Kit, any special treatment needed?

We are going to analyse the total bacterial community in Glacier snow ecology.

18 October 2016 7,866 0 View