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I want to isolate (preferably infectious) hepatitis C viral particles form infected patient's serum. I have reviewed some protocols which are based on PEG 8000, PEG 6000 with or without salt...
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I need an HIV replicons which lack genes necessary for virion release and spreading infection in cell culture but which can replicate intracellularly and may produce some of the HIV proteins.
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I have a purified enzyme. I need to test its activity. Enzyme requires ATP as energy currency. I have disodium salt of ATP (A2383). According to specs sheet if the solution will be prepared it...
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