Muhammad Ali

8 Questions 15 Answers 0 Followers

Questions related from Muhammad Ali

Any advice on the difference in the Ct value of reference gene in treated and control samples?

I am using 16S primers for qPCR. However I got different Ct values in may treated and control sample (30.08 and 28.85). Would it lead to considerable error in final results? In my opinion,...

23 July 2015 8,823 5 View

Is it possibile to determine genomic DNA contamination in cDNA sample on the basis of qPCR results?

Is there any possible way to find out genomic DNA contamination/residues in cDNA by analyzing results of qPCR? Can we get any information related to genomic DNA by Ct value?

27 June 2015 3,224 3 View

Which are experimentally proved Best house keeping genes to study gene expression in Pseudomonas syringae?

I need some information about house keeping genes to be used as internal control particularly for Pseudomonas syringae. Kindly let me know which house keeping genes are good to study gene...

22 January 2015 3,846 3 View

Which are the best expression vectors for Pseudomonas syringae?

I need some good expression vectors for Pseudomonas syringae for over expression of proteins and for the construction of complementary strain. along with that, I also need the source/link from...

30 September 2014 5,070 3 View

What software/database could be used for identification of pan genome/core genome of user's own sequence data?

1) The sequence data is in contigs form. 2) Please suggest windows based tools. 3) Important literature/source would also be appreciated.

29 September 2014 9,898 17 View

How gene knock out could be confirmed in bacteria?

Which techniques should be used to confirm that gene has been knocked out and why? What about DNA sequencing of gene of interest by flanking primers?

26 June 2014 8,233 3 View

Is it possible to identify a pathogenicity genomic island when a genome is present in contigs, and not as a finished complete chromosome?

We have a genome of laboratory bacterial strain. However, genome is at draft level and it is composed of nearly 200 contigs. Is it possible to identify PGI? If yes, which tools/softwares are easy...

26 June 2014 3,044 2 View

What should be the cut off criteria for E value, percentage identity, and query cover when blastp is used for identification of genes/homologs?

I want to identify virulent genes in my laboratory strain genome by using blastp tool. What should be criteria of E value and percentage identity? I shall be grateful if someone can send me some...

26 June 2014 1,552 4 View