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I am trying to purify my enzyme from plant source. I used 0-80% ammonium sulphate, can I use gel filteration using sephadex G-50 resin to desalt my sample instead of dialysis. As dialysis leads to...
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I am working on purifica of pectinmethylesterase enzyme. Many scientists have used mostly affinity chrom. For the same. I cannot find any paper where purification is done by ion exchange chrom. If...
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hello thr, I am trying to purify my enzyme by using IEC (anion) using biorad FPLC unit. I tried isocratic as well as gradient elution methods but getting four flat peaks out of which first two are...
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