Mugdha Dabir

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Questions related from Mugdha Dabir

How do I analyze structural changes in α-helices or ß-sheets by simple techniques?

I want to see the change in protein conformation after they are exposed to heating conditions. what are the techniques available? Please let me know a cost effective lab scale method.

14 February 2015 757 6 View

Instead of dialysis can I use sephadex G50?

I am trying to purify my enzyme from plant source. I used 0-80% ammonium sulphate, can I use gel filteration using sephadex G-50 resin to desalt my sample instead of dialysis. As dialysis leads to...

09 February 2015 1,828 4 View

Why is pectinmethylesterase enzyme purification done by affinity chromatography?

I am working on purifica of pectinmethylesterase enzyme. Many scientists have used mostly affinity chrom. For the same. I cannot find any paper where purification is done by ion exchange chrom. If...

09 February 2015 4,556 5 View

How to make the use of the ultrasonication technique cost effective?

I am working on food processing techniques and hence sing one of the method i.e ultrasound. But the main factor which comes is cost effectiveness. Can anyone guide me how to compete the...

07 February 2015 6,424 6 View