Can I set "20 uL reaction volume" in PCR cycler machine, while my actual sample is only 10 or 15 uL?

This is a stupid but quite technical question. We have a PCR machine that has 20 uL minimum reaction, so it can't be set into 15 or 10 uL in the machine. Is this going to affect the result...

23 March 2020 3,634 4 View