Mubin Tarannum

4 Questions 13 Answers 0 Followers

Questions related from Mubin Tarannum

Can anyone help with a protocol for preparing cells for Flow cytometry?

Hello. I want to use flow cytometry for the ROS production due to the exposure of Nanoparticles. My cells are adherent cell cultures. Please suggest a detaching solution and the concentration...

01 January 2015 8,671 7 View

Can someone suggest a protocol for preparing cell samples for flow cytometry analysis?

I am trying to find the ROS production in adherent cells after exposing them to stress causing agents, using flow cytometry. I am no sure how should I go about the sample preparation. Please help

11 November 2014 2,558 13 View

How to measure ROS, using the confocal imaging?

I am trying to measure ROS using confocal imaging. I tried to grow cells on cover slips. After 24 hours I add the stress causing agent, then after 24 hours I wash the cells with PBS. After washing...

09 September 2014 2,062 4 View

Is anyone familiar with collecting ROS measurements with a plate reader?

hello.. please help me with this. I do ROS analysis with microplate reader as well. i load the dye to cells after 24 hrs of their growth and wash them after 30 mins, add fresh media 1% FBS and...

09 September 2014 1,708 9 View