Mubin Tarannum

4 Questions 16 Answers 0 Followers

Questions related from Mubin Tarannum

Anyone familiar with LDH assay and MTT assay?

Hello I run both cell viability using MTT and membrane integrity using LDH analysis, but I find the same compound shows higher levels of LDH release compared to the cell viability data. Can anyone...

16 February 2015 1,282 19 View

Can someone suggest a protocol for preparing cell samples for flow cytometry analysis?

I am trying to find the ROS production in adherent cells after exposing them to stress causing agents, using flow cytometry. I am no sure how should I go about the sample preparation. Please help

15 November 2014 8,266 12 View

Is anyone familiar with collecting ROS measurements with a plate reader?

hello.. please help me with this. I do ROS analysis with microplate reader as well. i load the dye to cells after 24 hrs of their growth and wash them after 30 mins, add fresh media 1% FBS and...

27 September 2014 1,513 9 View

Is anyone familiar with ROS measurement using confocal microscopy?

I am trying to measure ROS using confocal imaging. I tried to grow cells on cover slips. After 24 hours, I add the stress causing agent, then after 24 hours I wash the cells with PBS. After...

21 September 2014 1,262 10 View