Mubin Tarannum

5 Questions 17 Answers 0 Followers

Questions related from Mubin Tarannum

Anyone familiar with LDH assay and MTT assay?

Hello I run both cell viability using MTT and membrane integrity using LDH analysis, but I find the same compound shows higher levels of LDH release compared to the cell viability data. Can anyone...

16 February 2015 1,326 19 View

What is the correlation between cell viability and cellular ROS production?

Hello. I performed ROS production analysis on A549 cells exposed to nanoparticles, I know that primary mechanism to be investigated for NP toxicity is the ROS production or oxidation stress and...

17 January 2015 979 10 View

Can someone suggest a protocol for preparing cell samples for flow cytometry analysis?

I am trying to find the ROS production in adherent cells after exposing them to stress causing agents, using flow cytometry. I am no sure how should I go about the sample preparation. Please help

15 November 2014 8,303 12 View

Is anyone familiar with collecting ROS measurements with a plate reader?

hello.. please help me with this. I do ROS analysis with microplate reader as well. i load the dye to cells after 24 hrs of their growth and wash them after 30 mins, add fresh media 1% FBS and...

27 September 2014 1,540 9 View

Is anyone familiar with ROS measurement using confocal microscopy?

I am trying to measure ROS using confocal imaging. I tried to grow cells on cover slips. After 24 hours, I add the stress causing agent, then after 24 hours I wash the cells with PBS. After...

21 September 2014 1,304 10 View